I am a postdoctoral fellow with the Garner lab employing high-resolution microscopy in live bacteria and archaea towards the understanding of the cell cycle fate. My aim is to collect quantitative data about cellular processes in real time through observation and perturbation of molecular dynamics on the nanoscale in living cells. As a member of the consortium, I’m focusing my studies towards developing imaging-based tools to observe the cell cycle of the archaea H. salinarum as a proof-of-concept for live-cell imaging in archaeal model organisms. In addition, I’m also interested in investigating the dynamics of archaeal cytoskeletal proteins and structures, honing in on homologs from the Asgard clade which I can express in heterologous systems. Imaging-based studies of these proteins will be coupled with in vitro polymer kinetics assays to better understand the mechanisms governing filament dynamics in this domain of life.
I am a postdoc in Ricardo Henriques’ lab with experience in developing hardware and novel analytical techniques for super-resolution microscopy. Currently, there is no commercial microscopy system available for live imaging of Archaea with sufficiently high spatio-temporal resolution to accurately observe processes such as cell division. To address this, I am building a microscope and developing analytical tools for high resolution live-cell imaging in Archaea-friendly conditions, i.e. 70-80ºC, low pH and minimal photo damage. Furthermore, the microscope will be capable of performing live-cell super-resolution imaging through structured illumination microscopy (SIM) and Super-Resolution Radial Fluctuations (SRRF); for fixed cells resolutions on the scale of tens of nanometres will be achievable using single molecule localization microscopy (SMLM) techniques.
I am a research fellow in Mohan’s group at the University of Warwick. My aim is to find Archaeal cytoskeletal proteins and identify their structure using spinning disk confocal microscopy. I am currently using fission yeast as a system to express and image Archaeal proteins. I believe we can re-construct and characterise complex Archaeal cytoskeleton networks by co-expressing multiple proteins using this genetically tractable organism.
Gabriel Tarrason Risa
I joined Buzz's team at the LMCB UCL in Autumn 2016 for an MRC funded PhD. My primary focus is the progression of cell division in Archaea. Using Sulfolobus as a model system, I look to characterise the order of events and roles of key division proteins as it appears during division. Furthermore, I look to explore the regulatory aspects of division in Archaea.